- Selcuk Journal of Agriculture and Food Sciences
- Vol: 17 Issue: 31
- The Effects of Azospirillum Brasilense and Two Rhizobium Species on The Nodulation of Bean (Phaseolu...
The Effects of Azospirillum Brasilense and Two Rhizobium Species on The Nodulation of Bean (Phaseolus Vulgaris L.) Cultivars
Authors : Mehmet Öğüt, Mustafa Kiliç, A Reşit Brohi
Pages : 5-12
View : 9 | Download : 14
Publication Date : 2003-06-25
Article Type : Research Article
Abstract :The objective of this study is to determine whether the synergistic effect of Azospirillum and Rhizobium double in-oculation on nodulation in common bean is additive with respect to bean cultivar. A pot experiment, designed as a generalized randomized block (bean cultivars) with 2 factors (Azospirillum brasilense Sp7 density and Rhizobium density) and 3 replications, was conducted. Rhizobium (a mixture of Rhizobium etli and Rhizobium tropici) inoculation at 106 cfu ml-1 increased all nodulation values (nodule number and dry weight in the first 5 cm of root system, the root system excluding the first 5 cm and total root) at 5 % significance level. However, increasing the Rhizobium density further did not change the nodulation pa-rameters except for the nodule number in the top 5 cm of root system at the 5 % significance level as per the LSD test. Azospirillum increased nodule weights (p<0.05). Based on the LSD tests, the best cultivar in terms of nodulation was şeker (p<0.05). The population of inoculated microbes probably reached an equilibrium level by time regardless of the initial cell density in inoculants. The plants supported only a limited number of nodules. Therefore, increasing the Rhizobium cell density in inoculant above certain levels is not suggested in terms of nodulation. The results of this study indicated that Azospirillum could provide the nodules with more carbon to function by affecting the plant growth.Keywords : Rhizobium etli, Rhizobium tropici, Azospirillum brasilense Sp7, Phaseolus vulgaris L.