- Middle Black Sea Journal of Health Science
- Vol: 8 Issue: 2
- YY1 and NFYA: Potential tr-KIT Specific Transcription Factors in Prostate Cancer
YY1 and NFYA: Potential tr-KIT Specific Transcription Factors in Prostate Cancer
Authors : Sercan Ergün, Ferda Ari, Erdal Benli, Diler Us Altay, Tevfik Noyan, Havva Erdem, Yeliz Kaşko Arici
Pages : 202-207
Doi:10.19127/mbsjohs.1001931
View : 26 | Download : 15
Publication Date : 2022-05-31
Article Type : Research
Abstract :Objective: Via the use of an alternative promoter, a truncated c-KIT protein (tr-KIT) of 30-50 kDa is generated, lacking extracellular and transmembrane domains. Moreover, over-expression of tr-KIT, a stronger activator than c-KIT, appears to be specific to prostate cancer (PCa). Also, Imatinib, a tyrosine kinase inhibitor, blocks the activity of full-length c-KIT but has no effect on tr-KIT in PCa. Tr-KIT has its own nuclear factor binding site. However, the transcription factors (TFs) binding to this region specific to tr-KIT are not known yet. This study was conducted to define the most potential TFs specific for tr-KIT via in silico analysis. Methods: Tr-KIT potential TF binding sequence was uploaded into Tfsitescan database. Five TFs with the highest potential binding to this sequence were selected. Transcriptomic data of LNCaP (PCa expressing tr-KIT), PC3 (PCa not expressing tr-KIT) and RWPE-1 (normal prostate) cell lines (GSM1647378, GSE36022 and GSM738189, respectively) from Gene Expression Omnibus (GEO) database were compared for gene expression levels of pre-defined potential tr-KIT specific TFs using DESeq package of R-program. Finally, two TFs having higher expression levels in both LNCaP and PC3 compared to RWPE-1 and higher expression levels in LNCaP compared to PC3 were detected. Results: Five TFs having the highest potential were selected as: YY1, c-MYB, IL8, NFYA and TCF3. Via in silico analysis performed, it was found that YY1 and NFYA have the highest potential to be tr-KIT specific TFs in PCa, among them. Conclusion: YY1 and NFYA TFs may take a role in formation of tr-KIT in PCa.Keywords : Prostate cancer, transcription factors, gene expression regulation