- Journal of Experimental and Clinical Medicine
- Vol: 35 Issue: 4
- Antiproliferative and apoptotic effects of Onobrychis albiflora extract on HCT- 116 cells
Antiproliferative and apoptotic effects of Onobrychis albiflora extract on HCT- 116 cells
Authors : Muhammet Volkan Bülbül, Hanefi Özbek, Ozlem Bahadir Acikara, Serkan Ozbilgin, Burcın Ergene Oz, Ekın Kurtul, Ilknur Keskin
Pages : 101-109
View : 14 | Download : 2
Publication Date : 2019-11-18
Article Type : Research
Abstract :In this study, the cytotoxic effects of the extracts of methanol: water (80:20) prepared from the above-ground parts of the varieties of Onobrychis albiflora Hub.-Mor., Onobrychis argyrea Boiss. subsp. argyrea, Onobrychis galegifolia Boiss. and Onobrychis tournefortii (Willd.) Desv. species to HCT-116 cells were investigated. With cytotoxicity analysis that with these species inhibitor concentrations (IC50) which resulted in a 50% reduction in the proliferation of HCT-116 cells were identified. In continuation of the study; the antiproliferative and apoptotic effects of Onobrychis albiflora extract on HCT-116 cells were evaluated by Caspase 3, Annexin V / PI Apoptosis / Necrosis analysis, Apopxin Green and 7-AAD Apoptosis / Necrosis analysis. The antiproliferative, apop- totic and necrotic effects of Onobrychis albiflora extract on HCT-116 cells also were determined.Keywords : HCT-116, FACS, HEK-293, NECROSİS, APOPTOSİS, COLON CANCER