- Journal of Scientific Reports-B
- Sayı: 008
- INVESTIGATION OF THE EFFECTS OF SOME PLANT VARIETIES ON TYROSINASE ACTIVITY AND ANTIOXIDANT PROPERTI...
INVESTIGATION OF THE EFFECTS OF SOME PLANT VARIETIES ON TYROSINASE ACTIVITY AND ANTIOXIDANT PROPERTIES
Authors : Duygu Yaman, Hatice Serin, Merve Çaliş, Gülnur Arabaci
Pages : 35-42
View : 34 | Download : 34
Publication Date : 2023-12-31
Article Type : Research
Abstract :Free radicals and reactive oxygen derivatives that cause damage to cells are effectively reduced by antioxidants and converted into products with less toxicity. Antioxidants must be taken into the body continuously to maintain living health. The enzyme tyrosinase is an oxidase that catalyzes the oxidation of tyrosine in the synthesis of melanin, the pigment that gives color to the skin. Over or under-expression of the enzyme can cause various skin problems and neurodegenerative damage in living things. This study aimed to determine the antioxidant capacity of rosemary and green tea, two important plants that we consume in our daily life in meals and as herbal tea, by determining the tyrosinase enzyme inhibition effects, total phenolic substance content, and DPPH free radical scavenging activity. The natural extraction method is preferred. According to this method, green tea and rosemary plants were dried and extracted with pure water by brewing method. The rosemary plant inhibited the tyrosinase enzyme at 750 μg/ml concentrations by 75% and the green tea plant at 1000 μg/ml concentration by 77%. Total phenolic contents were determined by the Folin-Ciocalteu method. Green tea (186 mg/GAE) was found to have the highest phenolic content. Antioxidant capacities were determined by the DPPH(1,1-diphenyl-2-picryl-hydrazil) method, and their free radical scavenging effects were determined. Trolox and BHT were used as positive controls in the DPPH method. Green tea and rosemary showed the highest DPPH free radical scavenging activity at 1000 μg/ml concentrationKeywords : Tirozinaz, Antioksidan, Fenolik Madde, Biberiye, Yeşil Çay