- Journal of Scientific Reports-A
- Issue: 047
- POTENTIAL of ENZYMATIC METHODS and BIOLOG ECOPLATE ANALYSIS for INVESTIGATION of MICROBIAL FUNCTIONA...
POTENTIAL of ENZYMATIC METHODS and BIOLOG ECOPLATE ANALYSIS for INVESTIGATION of MICROBIAL FUNCTIONALITY in AGRICULTURAL SOILS
Authors : Nilgün Poyraz, Suat Sezen, Mehmet Burçin Mutlu
Pages : 218-234
View : 20 | Download : 6
Publication Date : 2021-12-31
Article Type : Research
Abstract :Agricultural systems and applications affects the soil ecological conditions and microbial structure. However, it is important that the interventions do not disturb the balance and quality of the microbial content of the soil. For this reason, enzymatic methods and Biolog Ecoplate method were applied to research and evaluate the status of functional microbial diversity in four different agricultural soil samples (A, B, C, D). The results indicated that the pH, electrical conductivity, amounts of total nitrogen (N) and humidity were generally similar but calcium carbonate rates were higher in A and B agricultural soils. Soil enzymatic activity results showed some differences among the four different agricultural soils. The activity measurements of urease, phosphatase and dehydrogenase were high and results showed the differanties. Enzymatic activities and microbial populations correlated with each others and content of organic carbon. Evaluation of substrate utilization profiles and the diversity indices concluded that microbial community structure and composition were different related to various conditions. The average well color development (AWCD) which was calculated in the Biolog EcoPlate analysis showed some variations in the catabolic ability of four different agricultural soil samples’ microbial communities. Compared to other samples, C and D agricultural soil samples had a higher overall AWCD value. AWCD of C soil was significantly higher than in the others. Lowest used substrates were α- Cyclodextrin, α-Ketobutyric acid, β-Methyl-D-glucoside, α-D-Lactose and 2- hydroxybutyric acid. The most extensively used substrates were aminoacids and carbohydrate groups. These results indicates the degradation potential. With the Biolog EcoPlate and enzymatic measurements, changes in the microbial community in agricultural soils can be detected, and also agricultural management and application methods for tillage can be evaluated.Keywords : Dehydrogenase, urease, phosphatase, AWCD, Biolog Ecoplate, microbial activity