- Journal of Pharmaceutical Technology
- Vol: 3 Issue: 1
- Development and validation of an HPLC method for determination of quercetin
Development and validation of an HPLC method for determination of quercetin
Authors : Deniz Onan, Tilbe Çevikelli, Umay Merve Güven, Esra Demirtürk
Pages : 1-6
Doi:10.37662/jpt.2022.1000275
View : 11 | Download : 3
Publication Date : 2022-12-01
Article Type : Research
Abstract :Abstract This study validated a high performance liquid chromatography (HPLC) method for the determination of quercetin (QE) in microemulsion (ME) based gel formulations. The analyses were performed on a C18 column (150x4.6 mm, 5 m particle size) at room temperature with UV detection at 254 nm. The mobile phase was composed of methanol:water (65:35, v/v, 2% acetic acid) mixture, and flow rate was set to 1 mL/min. The method was validated according to the international guidelines with respect to linearity range, stability, limit of quantitation and detection, precision and accuracy. The method was linear within 5 – 100 µg/mL range with a correlation coefficient of 0.9995. The intra- and inter-assay precisions presented RSD values lower than 2%. The method reported is a fast and reliable HPLC method useful for QE determination in ME based gel formulations.Keywords : Quercetin, microemulsion based gel, validation, HPLC