- Journal of the Turkish Chemical Society Section A: Chemistry
- Vol: 8 Issue: 1
- Cytotoxic effects of Mannich bases via induction of caspase-3 pathway on human oral squamous cell ca...
Cytotoxic effects of Mannich bases via induction of caspase-3 pathway on human oral squamous cell carcinoma
Authors : Cem Yamali, Halise Inci Gül
Pages : 187-194
Doi:10.18596/jotcsa.817007
View : 10 | Download : 9
Publication Date : 2021-02-28
Article Type : Research
Abstract :In anticancer drug research, there is a need for the synthesis of compounds with selective cytotoxicity compared to the drugs in the market. The current study aimed to determine the cytotoxicities of the bis Mannich bases 1-9 towards human oral squamous cell carcinoma (OSCC). Mannich bases showed cytotoxicity in low micromolar in the range of 1.7-27 µM against OSCC cell lines. The compounds 5 with the highest potency selectivity expression (PSE) value (318.1) and 7 with the highest tumor selectivity (TS) values (TS1:11.2, TS2:15.8) showed promising selective cytotoxicity towards cancer cell lines. According to Western blot analysis, representative compound 7 induced the activation of caspase-3 in HSC-2 cells. These results may suggest that the apoptosis pathway may be one of the possible mechanisms of the actions and lead compound 7 can be subjected to further bioassays.Keywords : Mannich, anticancer, apoptosis