- Journal of the Turkish Chemical Society Section A: Chemistry
- Vol: 5 Issue: 1
- Electrochemical xanthine biosensor based on zinc oxide nanoparticles‒multiwalled carbon nanotubes‒1,...
Electrochemical xanthine biosensor based on zinc oxide nanoparticles‒multiwalled carbon nanotubes‒1,4-benzoquinone composite
Authors : Berna Dalkıran, Ceren Kaçar, Pınar Esra Erden, Esma Kılıç
Pages : 317-332
Doi:10.18596/jotcsa.307414
View : 13 | Download : 5
Publication Date : 2017-09-01
Article Type : Research
Abstract :Zinc oxide nanoparticles (ZnONPs), multiwalled carbon nanotubes (MWCNTs) and 1,4-benzoquinone (BQ) dispersed in chitosan (CS) matrix were used to construct a xanthine biosensor. Xanthine oxidase (XOx) was immobilized onto BQ-MWCNTs-ZnO–CS composite modified glassy carbon electrode (GCE) using glutaraldehyde as the crosslinking agent. The parameters of the construction process and the experimental variables for the biosensor were optimized. The xanthine biosensor showed optimum response within 10 s, and the sensitivity was 39.4 μA/mMcm 2 at +0.25 V (vs. Ag/AgCl). The linear working range of the biosensor was found to be 9.0×10 −7 - 1.1×10 −4 M with a detection limit of 2.1×10 -7 M. The biosensor exhibited good long-term stability and reproducibility. The presented biosensor was also used for monitoring the freshnesses of chicken and beef flesh.Keywords : Xanthine, biosensor, zinc oxide nanoparticles, carbon nanotubes, mediator