- Journal of International Environmental Application and Science
- Vol: 14 Issue: 1
- Determination of genotoxic effects in vitro of ZnOTiO2 nanoparticles on human peripheral lymphocytes
Determination of genotoxic effects in vitro of ZnOTiO2 nanoparticles on human peripheral lymphocytes
Authors : Yeşim Dağlioğlu, Huri Özkan Yilmaz, Orhan Yilmaz
Pages : 7-12
View : 9 | Download : 2
Publication Date : 2019-03-31
Article Type : Research
Abstract :The aim of this study was to examine the genotoxic effects of ZnOTiO 2 NPs using chromosome aberration (CA) and micronucleus (MN) tests in human peripheral lymphocytes. Cells were treated to ZnOTiO 2 NPs concentrations in the range of 12.5 to 125 µg/mL for 24 and 48 hours. In addition, it induced structural CA at all concentrations with ZnOTiO 2 NPs treatment. However, nanoparticles did not stimulate CA in a dose-dependent manner for 24 and 48 hours of treatment, and there were no statistically significant differences between both the administration time and the concentrations (P > 0.05). The formation of MN induced by ZnOTiO 2 NPs increased depending on concentration and dose. In particular, the highest 2 concentrations (100 and 125 µg/ml ) were observed to be significantly increased during the 24 and 48 hour treatment period (P > 0.05). Again, there is no significant difference in the occurrence of MN depending on gender (P > 0.05).Keywords : Chromosome aberration, ZnOTiO2 NPs, nanoparticles, nanotoxicology, micronucleus