- Journal of International Environmental Application and Science
- Vol: 11 Issue: 1
- Enterotoxemia in Albanian Zoo-Park Llama (Lama glama): Clostridium perfringens-type C was the Causat...
Enterotoxemia in Albanian Zoo-Park Llama (Lama glama): Clostridium perfringens-type C was the Causative Agent
Authors : Sulo Kotorri, Kastriot Korro, Rigers Bakiu, Pellumb Muhedini, Besa Gora
Pages : 42-46
View : 6 | Download : 2
Publication Date : 2016-03-30
Article Type : Research
Abstract :Clostridium perfringens causes enteric diseases in mammals, usually called enterotoxemia. C. perfringens was isolated from ileum and jejunum of dead llamas in one of zoo-parks in Tirana. Diagnosis of the disease was based on clinical sings, pathologically and also in isolation of the causes. Isolates colonies in agar blood were like dewdrops, smooth, gray, shiny and convex. In microscopic preparations colored and prepared with Gram technique were visible small bacillus, in sticks shape and Gram positive. Biochemical identification with API 20A kit valued isolates catalase and lecithinase positive, with hemolytic activity in agarblood from sheep with a dual zone of hemolysis. Isolates fermented producing gas and acid toward glucose, fructose lactose, sucrose and mannitol. Biological test of neutralization in white mice with antitoxins α and β, determined type C of C. perfringens as cause of llamas death, a result that was confirmed by ELISA kit. Besides treatment with drugs for pre-protection of llamas was used bivalent killed vaccine with types C and D of Clostridium perfringens .Keywords : Enterotoxemia, C.perfringens, API 20A, biological test of neutralization, ELISA, llamas