- Journal of International Environmental Application and Science
- Vol: 11 Issue: 2
- Comparative study for production and charcterization of D-Xylanase produced from locally isolate Asp...
Comparative study for production and charcterization of D-Xylanase produced from locally isolate Aspergillus niger by submerged and solid state fermentation
Authors : Ayat Adnan Abbas
Pages : 189-197
View : 4 | Download : 2
Publication Date : 2016-06-30
Article Type : Research
Abstract :Xylanase was pooled from Aspergillus niger that was cultivated on czepak dox with addition rice husk. Condition affecting xylanase production were assessed these included p H, temperature of cultivation, carbon source, nitrogen source, incubation period, and inoculums size for both types of culture. Results obtained were as follow ion exchange DEAD-Cellulose specific activity from solid state fermentation (І, ІІ) were (902.43,3666.6 U/mg), with yield (0.602,0.212%) and No. of fold (1.24,5.034). The specific activity from submerged fermentation ( P 1, P2) were (1861.76,910 U/mg), with yield (1.033, 0.531%) and No. of fold (5.570, 2.790). the optimum and stability p H for enzyme activity type (ІІ) produced by solid state fermentation (7.0 and 7.0) respectively. the optimum and stability temperature for enzyme type (ІІ) produced by solid state fermentation 50°C and 70 °C respectively. and the optimum and stability p H for enzyme activity type (P1) produced by submerged (7.0 and 7.0) respectively. the optimum and stability temperature for enzyme activity type (P1) produced by submerged fermentation 30 and 60 °C respectively.Keywords : xylanase, solid state fermentation, purification, submerged fermentatio