- Journal of International Environmental Application and Science
- Vol: 10 Issue: 4
- Optimum Conditions for LDPE Strips Biodegradation by Local Bacterial Isolates
Optimum Conditions for LDPE Strips Biodegradation by Local Bacterial Isolates
Authors : Amel Ali Hussain, Saad H. Khudhair, Ithar K. Al-mayaly
Pages : 399-407
View : 7 | Download : 3
Publication Date : 2015-10-30
Article Type : Research
Abstract :Optimum conditions (Temperature, pH and incubation time) were studied for selected bacterial isolates Pseudomonas fluorescens, Pseudomonas aeruginosa and Acinetobacter ursingii to biodegrade the low density polyethylene (LDPE) by using mineral salts medium (MSM) contains one pieces of LDPE strips (1cm × 5cm) using bacterial growth and amount of dissolved carbon dioxide as an indicator for ability of degradation. The results have been revealed that the above three bacterial isolates were shown high ability to degrade the LDPE strips after 15 days of incubation in liquid mineral salts medium pH 7 in 30 ˚ C, the growth was 0.351, 0.272 and 0.241, respectively, while the amount of dissolved CO 2 was 1.18, 1.05 and 0.95 g/l, respectively. The results of FTIR spectrum of LDPE strips which treated with three bacterial isolates were shown different changes in transmission bands for active groups of LDPE strips. Pseudomonas fluorescens isolates was shown the highest % of C-H group biodegradation (40.47%) compared with the other two isolates 35.89% and 30.55%, respectively.Keywords : LDPE biodegradation, optimum condition, CO2, FTIR