- Journal of Immunology and Clinical Microbiology
- Vol: 1 Issue: 3
- Characterization of lymphocyte infiltration in molar pregnancies
Characterization of lymphocyte infiltration in molar pregnancies
Authors : Jabbar Salman Hassan
Pages : 68-72
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Publication Date : 2016-12-30
Article Type : Research
Abstract :Background: In normal pregnancy around 30% of stromal cells in decidualised endometrium are leucocytes, lymphocyte subtypes may play a role in the pathogenesis of molar pregnancies. The aim of the study is t o investigate the pattern of infiltrating lymphocytes in paraffin embedded tissue sections obtained from molar pregnancies. Material and Methods: This study included thirty-five archived paraffin-embedded samples of molar pregnancies; divided into 16 incomplete and 19 complete hydatid mole. Then dual immunofluorescence staining was used for phenotyping of lymphocytic infiltrate (CD3- CD19) and (CD4-CD8). Independent sample t-test was used to compare the mean cell counts between different study groups. Results: There is higher T cells (CD3) infiltrate in complete hydatid mole than normal placental tissue (p=0.003) and not significant with incomplete hydatid mole (p=0.089). Plasma cells (CD19) were higher in both complete hydatid mole CHM (p=0.012) and incomplete hydatid mole iCHM (p=0.013). Cytotoxic cells (CD8) were none significant in all groups while Helper cells (CD4) were significantly higher in CHM (p=0.017) and iCHM (p=0.025). Conclusion: The higher tissue infiltration with plasma cells and helper cells in both CHM and iCHM highlight the importance of these cells in molar pregnancy pathogenesis.Keywords : Hydatid mole, lymphocytes infiltration, Complete hydatid mole, incomplete hydatid mole