- Journal of Apitherapy and Nature
- Vol: 1 Issue: 3 - 2nd Propolis Conference Special Issue
- Fatty Acid Analysis and Biological Activity of Jordanian Propolis
Fatty Acid Analysis and Biological Activity of Jordanian Propolis
Authors : Ashok K. Shakya, Shankar Katekhaye, Ghaleb A. Oriquat, Rajashri R. Naik, Rajashri R. Naik, Anant Paradkar, Hugo Fearnley, James Fearnley
Pages : 33-33
View : 10 | Download : 4
Publication Date : 2018-12-08
Article Type : Other
Abstract :Propolis is a resinous natural product collected by bees ( Apis mellifera ) from tree exudates which is widely used in folk medicine 1 . Reports on Jordanian Propolis reveal the presence of new chemical compound 4(Z)-1-3-dihydroxyeupha-7,24-dien-26-oic acid 2 along with other compounds like pinobanksin-3-O-acetate, pinocemberin, chrysin 3 and lignoceric acid 2 . The present study was carried out to investigate the fatty acid composition, antioxidant and xanthine oxidase inhibition activity of Jordanian Propolis, collected from Al-Ghour region. The hexane extract of Jordanian Propolis contains different fatty acids, which are reported first time, using GC-FID. The major fatty acid identified were palmitic acid (44.5%), Oleic acid (18:1 ∆ 9 cis, 24.6%) , Arachidic acid (7.4%), Stearic acid (5.4%), linoleic acid (18:2 ∆ 9-12 cis, 3.1% ), caprylic acid (2.9%), lignoceric acid (2.6%), cis -11,14-eicosadienoic acid (20:2 ∆ 11-14 cis, 2.4%), palmitoleic acid (1.5%), cis-11-eicosenoic acid (1.2%), α–linolenic acid (18:3 ∆ 9-12-15 cis, 1.1%), cis -13,16-docosadienoic acid (22:2 ∆ 13-16 cis, 1.0%), along with minor constituents like saturated fatty acids. Antioxidant properties of the hexane extract were determined via DPPH radical scavenging, β-carotene bleaching assay and NO scavenging assay. The extract produced significant antioxidant activity in-vitro . The extract also exhibit appreciable xanthine oxidase inhibitory activity in-vitro .Keywords : Propolis, Naturel product, DPPH, In vitro