- Journal of Istanbul Veterinary Sciences
- Vetexpo-2019, KHVD-2019 Special Issue
- Polymorphonuclear leukocyte isolation from venous blood of the dog
Polymorphonuclear leukocyte isolation from venous blood of the dog
Authors : Güneş KARAKURT, Kader YILDIZ
Pages : 52-52
View : 13 | Download : 3
Publication Date : 2020-03-31
Article Type : Research
Abstract :The aim of the current study was to neutrophil isolation from venous blood samples of healthy dogs. Venous blood samples were obtained from Venae cephalica of clinically healthy dogs (n:5) into heparinized tubes. The blood samples (2 mL) and Percoll dilutions (45%, 54%, 63%, and 72%) prepared with Hanks Balanced Salt Solution (HBSS) were layered into sterile tubes. After centrifuge, the polymorphonuclear leukocytes (PMN) were aspirated between 63% and 72% interfaces of the Percoll dilutions into tubes. The samples of PMN observed under light microscopy. Viability was detected microscopically after stained with trypan blue dye. Diff-Quick staining was used to detect neutrophil purity of the isolated PMN. In the present study, the neutrophils ratio was calculated as 92% of the isolated polymorphonuclear cells. The neutrophil viability was calculated as 98% of PMNs isolated from the venous blood samples of healthy dogs. In the present study, the Percoll gradient centrifugation (72%, 63%, 54% and 45%) is fast technic for isolation of the neutrophils from venous blood samples of dogs.Keywords : dog, venous blood