- İstanbul Journal of Pharmacy
- Vol: 48 Issue: 2
- Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD
Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD
Authors : Ceren EMİR, Nehir ÜNVER SOMER
Pages : 32-37
View : 8 | Download : 2
Publication Date : 2018-08-31
Article Type : Research
Abstract :DOI: 10.5152/IstanbulJPharm.2018.416835 In this study, a reversed-phase, high pressure liquid chromatography (RP-HPLC) method was applied in the quantitative analysis of lycorine in the aerial parts and bulbs of Galanthus alpinus Sosn. var. alpinus collected from Rize during the flowering and fruiting seasons. A simple method to extract lycorine from the plant specimens was performed, using pre-packed columns with diatomaceous earth (Extrelut®). The chromatographic separation was achieved employing an isocratic system with a mobile phase including trifluoroacetic acid-water-acetonitrile (0.01:95:5) measured at a flow rate of 1 mL/min using a diode array detector (DAD). The lycorine content in the bulbs during the flowering and fruiting seasons was detected as 0.01576 and 0.02351 %, respectively. Also, validation studies showed that the method was specific, accurate and precise.Keywords : Galanthus alpinus Sosn. var. alpinus, Amaryllidaceae, lycorine, HPLC-DAD