- International Journal of Secondary Metabolite
- Vol: 9 Issue: 1
- Comparison of three different protocols of alkaloid extraction from Glaucium corniculatum plant
Comparison of three different protocols of alkaloid extraction from Glaucium corniculatum plant
Authors : Fatma Gonca Koçanci, Serap Niğdelioğlu Dolanbay, Belma Aslim
Pages : 43-51
Doi:10.21448/ijsm.980171
View : 8 | Download : 2
Publication Date : 2022-03-10
Article Type : Research
Abstract :Alkaloids, plant secondary metabolites, have a wide variety of biological effects. For this reason, the extraction of alkaloids from plants is of strategic importance. Different extraction protocols for the extraction of alkaloids from plants have been described by many authors. The objective of this study was to compare the efficacy of three different protocols for the extraction of alkaloids from Glaucium corniculatum. This article compares the Soxhlet and ultrasonication protocol, used in previous studies, to a modified Soxhlet protocol. While the alkaloid amount in the extract was determined by the spectrophotometric method, the qualitative estimation of the compounds in the extract was determined by Gas chromatograph-Mass spectrometer (GC-MS). The alkaloid amount and diversity in the extract, obtained through the recommended modified Soxhlet protocol, were higher than that of any extract obtained through other protocols. Thus, a new modified alkaloid extraction protocol was established, which is better than the other protocol.Keywords : Alkaloid, Extraction, Glaucium corniculatum, Ultrasonication, Soxhlet