- International Journal of Chemistry and Technology
- Vol: 6 Issue: 2
- Cytotoxic, apoptotic, and necrotic effects of silver nanoparticles biosynthesized using Origanum maj...
Cytotoxic, apoptotic, and necrotic effects of silver nanoparticles biosynthesized using Origanum majorana extract
Authors : Ramazan Erenler, Esma Nur Geçer, Büşra Moran Bozer
Pages : 142-146
Doi:10.32571/ijct.1187754
View : 27 | Download : 10
Publication Date : 2022-12-31
Article Type : Research
Abstract :application. Anticancer activity of Origanum majorana extract and silver nanoparticles was investigated by MTT assay using human pancreatic adenocarcinoma cell lines (Capan-1), mouse normal fibroblast cell lines (L929), and human colon adenocarcinoma cell line (Caco-2). AgNPs@Om exhibited the excellent cytotoxic effect on Capan-1 cell lines with the cell viability of 29.17% and 25.70%, 22.49%, 21.16% at 1.0, 0.5, 0.25, 0.125 μg/mL respectively. However, the extract was determined to show moderate activity. A considerable fall in the viable cell number in Caco-2 cell lines was observed after the treatment of AgNPs@Om. The viable cells of Caco-2 cell lines were detected as 32.0% at 1.0 μg/mL with the treatment of nanoparticles. Yet, the viable Caco-2 cells were determined as 48.2% with the treatment of O. majorana extract. Both extract and nanoparticles had no impact on the viability of non-tumor cells (L929). Moreover, nanoparticles induced apoptosis. Consequently, AgNPs@Om may be a promising anticancer drug candidate.Keywords : Silver nanoparticles, natural products, Origanum majorana, anticancer activity.