- Hacettepe Journal of Biology and Chemistry
- Vol: 49 Issue: 1
- In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibrobl...
In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells
Authors : Esin Akbay, Y. Doruk Aracagök, Ali Hakan Göker, Mehmet Ali Onur, Nilüfer Cihangir
Pages : 25-36
Doi:10.15671/hjbc.680564
View : 15 | Download : 3
Publication Date : 2021-01-01
Article Type : Research
Abstract :The aim of this in vitro study was assessment of the cytotoxic level of 6-O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and 6-O-desmethylnaproxen in different concentrations for 24- and 48- hours. Cell viability was tested by MTT, while apoptosis was determined by AO/PI double staining method. The control group was free from any agent (DMEM only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of 6-O-desmethylnaproxen (0.7 µg/mL) while none of naproxen concentration was caused 50% cell dead. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.Keywords : Cytotoxicity, naproxen, 6-O-desmethylnaproxen, in vitro