- Gazi University Journal of Science Part A: Engineering and Innovation
- Vol: 9 Issue: 4
- Effects of Different Culture Media Compositions on In Vitro Micropropagation from Paradox Walnut Roo...
Effects of Different Culture Media Compositions on In Vitro Micropropagation from Paradox Walnut Rootstock Nodes
Authors : Cem Dirlik, Hacer Kandemir, Nurberat Çetin, Senem Şen, Begüm Güler, Aynur Gürel
Pages : 500-515
Doi:10.54287/gujsa.1194822
View : 12 | Download : 4
Publication Date : 2022-12-31
Article Type : Research
Abstract :The aim of this study is to establish an effective protocol for in vitro micropropagation from node explants of the \'Paradox\' (Juglans regia x J. hindsii) rootstock. In the first stage of the study, the node explants were cultured in semi-solid media after being exposed to different sterilization methods. The most effective sterilization method was observed as ST2 with the lowest darkening rate (4 %). According to the results, SP4 medium provided the highest number of shoots per unit explant (1.05 shoots/explant) and 100 % shoot formation, SP2 medium provided the best mean shoot length (1.6 cm) and the mean number of nodes per unit explant (7.95 nodes/explant). Best root induction was provided from ½ Modified MS medium containing 4 mg/L IBA with 30 g/L sucrose. For root induction explants were retained under dark conditions for 7 days at 24±2oC. In order to ensure root formation and elongation, shoot explants with root induction cultured in a 16-hour photoperiod (3500 lux) for 21 days in different nutrient media containing 2.4 mg/L Gelrite, hormone-free and mixed with vermiculite (1:1 v/v) and was rooting rate is 45.45 % in ¼DFe medium.Keywords : Micropropagation, Walnut, Juglans regia L., Paradox, In vitro