- Gazi University Journal of Science
- Cilt: 36 Sayı: 4
- Magnetic Separation of Micro Beads and Cells on a Paper-Based Lateral Flow System
Magnetic Separation of Micro Beads and Cells on a Paper-Based Lateral Flow System
Authors : Muhammad Fuad Farooqi, Kutay Içöz
Pages : 1538-1551
Doi:10.35378/gujs.1146050
View : 273 | Download : 476
Publication Date : 2023-12-01
Article Type : Research
Abstract :Paper based lateral flow systems are widely used biosensor platforms to detect biomolecules in a liquid sample. Proteins, bacteria, oligonucleotides, and nanoparticles were investigated in the literature. In this work we designed a magnetic platform including dual magnets and tested the flow of micron size immunomagnetic particles alone and when loaded with cells on two different types of papers. The prewetting conditions of the paper and the applied external magnetic field are the two dominant factors affecting the particle and cell transport in paper. The images recorded with a cell phone, or with a bright field optical microscope were analyzed to measure the flow of particles and cells. The effect of prewetting conditions and magnetic force were measured, and it was shown that in the worst case, minimum 90% of the introduced cells reached to the edge of the paper. The paper based magnetophoretic lateral flow systems can be used for cell assays.Keywords : Image processing, Magnetophoresis, Cancer cells, Bright-field optical microscope