- Gazi University Journal of Science
- Vol: 26 Issue: 2
- Spectrofluorimetric Determination of Diiodohydroxyquinoline in Presence of Metronidazole in Pharmace...
Spectrofluorimetric Determination of Diiodohydroxyquinoline in Presence of Metronidazole in Pharmaceutical Formulation and Spiked Human Plasma
Authors : Y Aboul-enein, Nouruddin Ali, Mohammed Gamal, Mohammed Abdelkawy
Pages : 141-149
View : 10 | Download : 4
Publication Date : 2013-07-05
Article Type : Research
Abstract :Accurate and sensitive spectrofluorimetric method was developed for determination of diiodohydroxyquinoline in presence of metronidazole. In this method the native fluorescence of diiodohydroxyquinoline in water solvent at λ =495 nm when excitation was at 250 nm is used for its determination. Linear correlation was obtained in theconcentration range of 400 to 900 ng mL-1. The proposed method was successfully applied for determination ofdiiodohydroxyquinoline in bulk powder with mean accuracy of 100.21±1.13 or in spiked human plasma with mean accuracy of 100.53±1.42 without interference of metronidazole.Keywords : spectrofluorimetry, Diiodohydroxyquinoline, Metronidazole