- Gazi University Journal of Science
- Vol: 16 Issue: 2
- THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITI...
THE EFFECTS OF 20-HYDROXYECDYSONE ON HEMOCYTES OF GALLERIA MELLONELLA (LEPIDOPTERA) IN VITRO CONDITIONS
Authors : Savaş Izzetoğlu, Sabire Karaçali
Pages : 233-238
View : 21 | Download : 5
Publication Date : 2010-08-06
Article Type : Other
Abstract :ABSTRACT The effects of different concentrations of 20-hydroxyecdysone (20-E) on hemocytes of Galleria mellonella were investigated in vitro. Hemocyte culture was performed in Grace's insect medium with fetal calf serum. The hormone was added to the cultures at two different concentrations. During a period of three days, the cells (plasmatocytes, granulocytes and total cell number) were counted under an inverted microscope. Obtained numbers were evaluated with one way variance analysis (ANOVA). Hormone treatment led to a dose-dependent decrease in total cell and granulocyte number. The number of plasmatocytes, which are the predominant cells for phagocytose activity in immune response, was enhanced by hormone treatment. Key Words: 20-hydroxyecdysone, hemocyte, in vitro, Galleria mellonella, insectKeywords : 20-hydroxyecdysone, hemocyte, in vitro, Galleria mellonella, insect