- Gazi University Journal of Science
- Vol: 31 Issue: 4
- Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane
Lectin Histochemistry of Agrotis segetum Midgut Cells and Peritrophic Membrane
Authors : Naznoosh Shomali–moghaddam, Nursel Gul, Ebru Gokalp Ozkorkmaz, Aydin Ozluk
Pages : 1022-1031
View : 18 | Download : 5
Publication Date : 2018-12-01
Article Type : Research
Abstract :Midgut cell and peritrophic membranes of grain pest Agrotis segetum larvae were examined by light microscope. Six biotinylated lectins were used in the assays by applying histochemical methods (Avidin-Biotin-Peroxidase). The aim of this study was to find lectins binding on peritrophic membranes if they were available as insecticidal agents. Results of the study indicated that lectins BPA ( Bauhinia purpurea ) and GS-I ( Griffonia simplicifolia ) strongly stained whereas, PNA (Pea Nut Agglutinin) and UEA-1 ( Ulex europaeus ) moderately stained the tissues. However, WGA (Wheat Germ Agglutinin) and Con-A ( Canavalia ensiformis ) stained the tissues weakly. The common feature of two binding proteins, BPA and GS-I lectin was binding to D- galactose. Our examinations revealed that D-galactose mostly exists in A. segetum larvae midgut cell membranes and peritrophic membrane and this means BPA and GS-I lectins can be used as insecticidal agents against A. segetum .Keywords : Agrotis segetum larvae, Biotinylated lectins, Midgut cells, Peritrophic membrane