- Gazi University Journal of Science
- Vol: 29 Issue: 2
- PREPARATION OF A NEW URIC ACID BIOSENSOR WITH IMMOBILIZATION OF URICASE UPON POLYPYRROLE-PARATOLUENE...
PREPARATION OF A NEW URIC ACID BIOSENSOR WITH IMMOBILIZATION OF URICASE UPON POLYPYRROLE-PARATOLUENE SULPHONATE FILM
Authors : Servet Çete, Gökçen Karpuz, Ahmet Yaşar
Pages : 293-301
View : 10 | Download : 4
Publication Date : 2016-06-20
Article Type : Other
Abstract :In this study, a new amperometric biosensor based on immobilized uricase was developed for the determination of uric acid. Polypyrrole - p -toluene sulphonate film was prepared on the surface of a platinum (Pt) electrode by electropolymerization of pyrrole in the presence of p -toluene sulphonate as an anionic dopant. Uricase enzyme were immobilized in polypyrrole - p -toluene sulphonate via the entrapment method. Determination of uric acid was performed by oxidation of enzymically generated H 2 O 2 at 0.3 V. Some factors that affect response current were studied such as temperature, pH and substrate concentration. Operation stability and storage stability of the biosensor was determined. The biosensor retained 76.6% of its initial performance after 30 assays and it lost 58% of its initial performance after 60 days. Effects of interferants on the current response of the biosensor were examined. The performance of the biosensor was measured in serum of healthy individuals.Keywords : Uric acid, uricase, biosensor, polypyrrole, paratoluensulphonate