- Eurasian Journal of Forest Science
- Vol: 7 Issue: 1
- TLC and GC-MS analysis of petroleum ether fraction of fermented wood "Nikhra" of Acacia se...
TLC and GC-MS analysis of petroleum ether fraction of fermented wood "Nikhra" of Acacia seyal
Authors : Abdalbasit Mariod
Pages : 23-29
Doi:10.31195/ejejfs.472038
View : 8 | Download : 3
Publication Date : 2019-02-28
Article Type : Research
Abstract :Aim of this study to detect organoleptically Acacia seyal fractions ( petroleum ether, chloroform, methanol and aqueous) fermented wood "Nikhra " of the fractions accumulating the strongest sweet fragrance these fragrances were mainly accumulated in the petroleum ether fermented wood "Nikhra " fraction and analysis it by using chromatographic and spectroscopic analysis. Petroleum ether fermented wood "Nikhra " fraction analysis with TLC and spray TLC with vanillin H 2 SO 4 (pink) (C1, C2,C3,C4,C5,C6,C7), Rf values (0.92, 0.86, 0.71, 0.64, 0.57, 0.50, 0.36) ,were expected to be phenolic, with vanillin HCL (red ) compounds spots (C5,C6,C7) with Rf values (0.57, 0.50, 0.37) was expected to be catechin and with vanillin H 3 PO 4 , blue-violet zones compounds spots (C6, C7), with Rf values ( 0.50, 0.36), were expected to be lignans. Petroleum ether fermented wood "Nikhra " fraction was divided into two types of compounds classes aromatic and non aromatic by http://research.easybib.com and hence compounds were classified to phenolics and terpenoids compounds by GC/MS. Fragrant aromatics or terpenoids were targeted in this part of study. GC-MS analysis gave a spectrum of fragrance aromatic compounds (phenolics) in the petroleum ether fermented wood "Nikhra " fractions of A.seyal , was Petadecanoic acid(5.64%) and Tetracosamethyl-cyclododecasiloxane(4.17%) total fragrance aromatic compounds(44.57%), and main terponoids compounds was Octadecanoic acid(2.52) % total fragrance aromatic compounds(11.87%).Keywords : Acacia seyal, Gas Chromatography (GC)