- Eurasian Journal of Forest Science
- Vol: 6 Issue: 3
- TLC and GC-MS analysis of fermented wood "Nikhra" petroleum ether fraction of Combretaceae...
TLC and GC-MS analysis of fermented wood "Nikhra" petroleum ether fraction of Combretaceae spp. Combretum hartmannianum and Terminalia laxiflora
Authors : Noha Fadle, Abdalbasit Mariod, Hiba Abdel Rahman Ali, Alfatih Ahmed Hasan
Pages : 1-7
Doi:10.31195/ejejfs.417112
View : 17 | Download : 4
Publication Date : 2018-10-26
Article Type : Research
Abstract :This study aims to analysis fractions ( petroleum ether, chloroform, methanol and aqueous) fermented wood Nikhra of Combretaceae spp ( Combretum hartmannianum and Terminalia laxiflora ) analysis it by using chromatographic and spectroscopic analysis. Petroleum ether Nikhra fraction analysis with TLC and spray TLC with vanillin H 2 SO 4 (pink) (B1, B2), Rf values (0.84, 0.81), were expected to be phenolic, with vanillin HCL (red ) compounds spots (B2) with Rf values (0.81) was expected to be catechin and with vanillin H 3 PO 4 , blue-violet zones compounds spots (A1, A2, A3), (B1, B2, B3, B4), with Rf values (0.88, 0.78, 0.67), (0.84, 0.81, 0.67, 0.59), respectively were expected to be lignans. Petroleum ether fermented wood "Nikhra " fraction was divided into two types of compounds classes aromatic and non aromatic by and hence compounds were classified to phenolics and terpenoids compounds by GC/MS. Fragrant aromatics or terpenoids were targeted in this part of study. GC-MS analysis gave a spectrum of fragrance aromatic compounds (phenolics) in the petroleum ether Nikhra fractions of T. laxiflora, was Lup-20(29)-en-3-ol, acetate, (3β) and Tetracosamethyl-cyclododecasiloxane, main terponoids compounds was eicosamethylcyclodecasiloxane. Main fragrance aromatics compounds in the petroleum ether Nikhra fraction of C. hartmannianum was 2-tert-Butyl-5-(hydroxtmethyl)-4-formylfuran, and main terponoids compounds was Tetracosamethylcyclododecasiloxane.Keywords : Combretaceae, Combretum hartmannianum, Terminalia laxiflora, Gas Chromatography