- Ekin Journal of Crop Breeding and Genetics
- Vol: 3 Issue: 1
- Molecular Characterization of Some Triticale Cultivars in Turkey
Molecular Characterization of Some Triticale Cultivars in Turkey
Authors : Merve Carikci
Pages : 61-65
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Publication Date : 2017-01-31
Article Type : Research
Abstract :Triticale has gained importance as an alternative crop to solve the nutritional problems of the rapidly increasing world population. Triticale gains the yield potential of durum wheat and adaptation of rye to cold, drought and marginal soil conditions in itself. It is also known that triticale is resistant to many diseases and pests. In this study, it was aimed to determine the genetic characterization of some spring and facultative triticale varieties registered in Turkey with the ISSR-PCR technique which is one of the molecular marker methods. Polymorphic and monomorphic band images were formed from 16 primers as a result of analysis of 20 primers in 5 registered triticale isolates, and 71.1% polymorphism was obtained. Four primers did not show any amplification in the triticale cultivars. Triticale cultivars were mainly divided into three groups. Alperbey and Tacettinbey which were in the first group, differed by 28% and Mehmetbey and Ayşehanım in the second group differed by 37%. In addition, EgeYıldızı in the third group differed 48% from Alperbey, 45% from Tacettinbey, 49% from Mehmetbey and 38% from Aysehanım. The genetic distance values between cultivars of triticale ranged from 0.509 to 0.712. These results obtained from ISSR-PCR method will provide significant contributions to the triticale breeding programsKeywords : Triticale, molecular characterization, polymerase chain reaction, PCR, ISSR, Inter-Simple Sequence Repeat