- Ziraat Fakültesi Dergisi
- Vol: 10 Issue: 2
- Bazı Arpa (Hordeum vulgare L.) Genotiplerinin Genetik Farklılığının RAPD ve ISSR Analizleri ile Beli...
Bazı Arpa (Hordeum vulgare L.) Genotiplerinin Genetik Farklılığının RAPD ve ISSR Analizleri ile Belirlenmesi
Authors : Murat Olgun, Zekiye Budak Başçiftçi, N. Gözde Ayter, Metin Turan, Onur Koyuncu, Murat Ardiç, Güleray Ağar, Engin Takil
Pages : 102-109
View : 15 | Download : 2
Publication Date : 2015-09-01
Article Type : Other
Abstract :The objective of this study was to determine genetic distance between barley genotypes based on RAPD and ISSR analyses. The number of amplified bands of genotypes in the primers ranged 4-11 in RAPD and 7-11 in ISSR. The most polymorphic bands in primer/primer combinations were obtained from OPA-04, OPA-13 and OPH-17 in RAPD, and UBC-808, UBC-820 and UBC-872 in ISSR. Results showed that average polymorphic rate was 86.5%. Performances of cultivars in groups asist possibility in creating genetic variability in barley cultivar development. RAPD and ISSR methods are useful for evaluation of genetic diversity and could be safely used to determine the genetic relationships in barley genotypes.Keywords : Arpa, genetik ayrışma, DNA ektrasyonu, genetik poliforfizm, RAPD ve ISSR