- Türk Doğa ve Fen Dergisi
- Vol: 12 Issue: 1
- Purification and Charcterızatıon Glutathione S-Transferase Enzyme From Chicken Liver
Purification and Charcterızatıon Glutathione S-Transferase Enzyme From Chicken Liver
Authors : Hakan Yilmaz, Mehmet Çiftci, Yusuf Temel
Pages : 117-124
Doi:10.46810/tdfd.1242764
View : 12 | Download : 2
Publication Date : 2023-03-27
Article Type : Research
Abstract :In this study, the glutathione S-transferase enzyme (GST; EC 2.5.1.18) was purified with 8.35 EU/mL specific activity, 24.56 times 8% yield, from chicken liver, using ammonium sulfate precipitation and glutathione-agarose affinity chromatography. In order to control the purity of the enzyme, SDS-PAGE was performed and a single band was obtained. The molecular mass of the subunit was calculated as approximately 30.9 kDa. In addition, the optimum pH value of the enzyme (8.5 in Tris-HCl); optimum ionic strength (150 mM with Tris-HCl); optimum temperature (70 oC); stable pH value (8.5 with Tris-HCl) was determined. The KM value for the GSH substrate of the enzyme was 0.802 mM, the Vmax value was 1.833 EU/mL; For CDNB, the KM value was calculated as 3.6 mM and the Vmax value was calculated as 2.829 EU/mL.Keywords : Glutatyon S-transferaz, tavuk karaciğeri, saflaştırma., Glutathione S-transferase, chicken liver, purification.