- Tıp Fakültesi Klinikleri Dergisi
- Vol: 2 Issue: 1
- Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplan...
Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti
Authors : Özer Akgül, Hayriye Kirkoyun Uysal, Ilker Tosun, Burcu Sapmaz, Reyhan Çalişkan, A. Canan Yazici Güvercin, Sinem Öktem, Özgür Kurt, Y. Ali Öner
Pages : 17-23
View : 17 | Download : 1
Publication Date : 2019-01-31
Article Type : Research
Abstract :Objective: Intestinal parasitic infections are among the prominent public health concerns in patients with suppressed immune function. Material and methods: Here we examined stool specimens by microscopy and polymerase chain reaction (PCR) to evaluate the infection of five principal protozoans ( Cryptosporidium spp., Giardia spp., Entamoeba histolytica , Blastocystis spp. and Dientamoeba spp.) among 90 renal transplant recipient patients (RTP) in comparison with 90 healthy individuals (HI) from Turkey. Results: The overall frequency of any parasites was 17.2% (31/180) with microscopy and 51.7% (93/180) with PCR. Because of its high sensitivity, PCR was compared with microscopy in terms of the accuracy of detecting intestinal parasites, and the agreement was found to be inadequate (κ= 0.217; p<0.001). Multiparasitism (90.9%), Cryptosporidium spp. (84.6%) and Giardia spp. (74.1%) were the most frequent agents in RTP, respectively (p<0.001). Conclusions: This is the first study performed in Turkish reporting the prevalence of five intestinal parasites with PCR techniques among this group and seeks to provide a basis for future studiesKeywords : Intestinal parasites, Transplantation, PCR, Microscopy