- Tarım Bilimleri Dergisi
- Vol: 23 Issue: 3
- Comparison of Microtubule Organization in Arabidopsis thaliana TUBGFP and MBD-GFP Mutants Exposed to...
Comparison of Microtubule Organization in Arabidopsis thaliana TUBGFP and MBD-GFP Mutants Exposed to UV-B Radiation
Authors : Dongjing Ma, Huize Chen, Rong Han
Pages : 328-334
Doi:10.15832/ankutbd.447642
View : 13 | Download : 6
Publication Date : 2017-09-01
Article Type : Research
Abstract :Microtubule organization was compared between the Arabidopsis thaliana TUB-GFP and MBD-GFP mutants. Plant height and primary root length were measured, and microtubule dynamics were examined by confocal laser scanning microscopy after UV-B radiation to reveal changes in microtubules. Damage caused by UV-B was comparable between transgenic lines and wild-type plants, although transgenic lines were more sensitive to UV-B than the wild-type. Spots and depolymerization of microtubules were detected in both TUB-GFP and MBD-GFP plants; however, MBD-GFP showed better adaptation of changes induced by UV-B treatment. These results indicated that UV-B inhibits the growth and development of transgenic lines, and the inhibitory effects might result from changes in microtubules, as determined by comparison between the TUB-GFP and MBD-GFP lines.Keywords : Microtubules, UV-B radiation, Arabidopsis thaliana TUB-GFP, Arabidopsis thaliana MBD-GFP