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- THE ANTICANCER ACTIVITY OF JUGLONE VIA INDUCING PROGRAMMED CELL DEATH IN PANCREATIC CANCER CELLS
THE ANTICANCER ACTIVITY OF JUGLONE VIA INDUCING PROGRAMMED CELL DEATH IN PANCREATIC CANCER CELLS
Authors : Dudu Erkoç Kaya, Duygu Dursunoğlu
Pages : 321-332
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Publication Date : 2023-09-21
Article Type : Research
Abstract :Aim: Pancreatic cancer (PC), has a poor prognosis with its silent course until the advanced level without any symptoms. Additionally, currently limited treatment options makes developing alternative treatment strategies and agents mandatory. In researches conducted to investigate new therapeutic agents for cancer natural products are main sources. Among all, Juglone, a natural naphthoquinone, stands out with its cytotoxic effect in cancer cells, induction of apoptosis and autophagy, inhibition of antiangiogenesis and cancer cell migration. Thus, in our study, we aimed to investigate the anticancer activity and the apoptotic effects of juglone on BxPC-3 Pancreatic cancer cells. Method: To evaluate the apoptotic effects; proapoptotic Bax, antiapoptotic Bcl-2 and an important inhibitor of apoptosis Birc5 (Survivin) gene expressions were determined by qPCR analysis. We also confirmed the results for apoptosis by performing immunofluorescence analysis using Annexin V-FITC. Findings: According to qPCR analysis, juglone induced statistically significant 2.24, 1.57, 2.43 fold increases of Bax gene the expressions at 10, 15 and 20µM doses, respectively. We detected low fold increases after juglone treatments for Bcl-2 gene expression at other treatments except 20µM dose. This changes were not statistically significant. Juglone decreased survivin gene expression at all treatment doses while 2.39 fold decrease at 20 μM doses of juglone was statistically significant. Also, we confirmed clearly by the immunofluorescence analysis that juglone increased apoptosis dose-dependently in BxPC-3 cells. Results: Taken together all the results of gene expression and immunfloresence analysis our study suggests that juglone shows anticancer activity by inducing apoptosis possibly enhancing intrinsic apoptotic pathway in Pancreatic cancer cells. Keywords: juglone, pancreatic cancer, Bax gene, Bcl-2 gene, survivin gene, apoptosis,Keywords : JUGLON, PANKREAS KANSERİ, APOPTOZ, BAX GENİ, BCL-2 GENİ, SURVIVIN GENİ