- Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi
- Vol: 22 Issue: 6
- Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats
Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats
Authors : Fatma Özdemir, Seza Arslan
Pages : 1674-1682
Doi:10.16984/saufenbilder.395016
View : 17 | Download : 8
Publication Date : 2018-12-01
Article Type : Other
Abstract :The genus Bacillus is frequently found in soil, water and food. Bacillus cereus and Bacillus anthracis are the main pathogens causing foodborne diseases and serious infections in humans. A total of 52 Bacillus spp. from meat samples was tested for determination of biofilm production, antimicrobial resistance pattern and beta-lactamase activity. The 24 (46.1%) Bacillus isolates were found to be for biofilm production. Of the 24 (46.1%) biofilm producer Bacillus isolates, 13 (25%), 6 (11.5%) and 5 (9.6%) were considered as strong, moderate and weak biofilm producer, respectively. The most common species for the production of biofilm was Bacillus thuringiensis (80%). Antimicrobial disk susceptibility tests of Bacillus spp. revealed high resistance to ampicillin (84.6%) followed by penicillin (75%), cefepime (34.6%), and cefoxitin (26.9%). A multidrug resistance to at least 3 or more antimicrobials was observed in the 25 isolates (48.1%). All Bacillus spp. were sensitive to vancomycin, gentamicin, amikacin, ciprofloxacin, and imipenem. The susceptibility rate to streptomycin, chloramphenicol, and trimethoprim-sulphamethoxazole was 94.2%. Among the isolates, the 6 (11.5%) isolates were found to be sensitive to all antimicrobial agents tested. Besides, only one isolate from meat was found to be positive for beta-lactamase test. The existence of biofilm production as a virulence factor and of multidrug resistance in bacteria isolated from food should not be underestimated in terms of food safety, public health and economic concerns.Keywords : Bacillus spp., biofilm production, antimicrobial resistance, beta-lactamase