- Sağlık Bilimlerinde İleri Araştırmalar Dergisi
- Cilt: 6 Sayı: 2
- COMPARISON OF THE IMMUNOMODULATORY PROPERTIES OF THE CORD BLOOD AND WHARTON’S JELLY DERIVED MESENCHY...
COMPARISON OF THE IMMUNOMODULATORY PROPERTIES OF THE CORD BLOOD AND WHARTON’S JELLY DERIVED MESENCHYMAL STEM CELLS
Authors : Ayşe Erol, Figen Abatay Sel, Mediha Süleymanoğlu, Gökhan Demirayak, Dürdane Serap Kuruca, Fatma Savran Oğuz
Pages : 136-142
Doi:10.26650/JARHS2023-1216719
View : 53 | Download : 63
Publication Date : 2023-06-26
Article Type : Research
Abstract :Objective: Mesenchymal stem cells (MSCs) are promising as a treatment option for many immune-related diseases due to their immune regulatory properties. Wharton’s Jelly (WJ-MSC) and cord blood (CB-MSC) have recently received more attention than the other MSC sources. In this study, it was aimed to investigate the difference in the mechanisms of the immunological effects of WJ-MSCs and CB-MSCs. Material and Methods: The intracellular cytokine levels of peripheral blood mononuclear cells (PBMC) and CD4+ T cells before and after MSC co-culture (Interleukin-4 (IL-4), Interferon-γ (IFN-γ), and Interleukin-17 (IL17)) were determined by flow cytometry. At the same time, tumor growth factor (TGF)-β, IL-4, IL-17, IFN-γ supernatant cytokine levels were measured by ELISA. In the study, incubation times of 24 hours and 72 hours were applied with co-culture MSC/PBMC ratios of 1/5 and 1/10. Results: Our data showed that WJ-MSCs and CB-MSCs have different morphological features, proliferation capacities, proliferation times and immunomodulating abilities. One of the cytokines of IFN-γ decreased significantly at both 1/5 and 1/10 ratios in the cell at 24 hours and increased significantly at 72 hours after CB-MSC/PBMC co-culture compared to the level after WJ-MSC/PBMC co-culture (p<0.05). Unlike for IL-17 cytokine, the intracellular level decreased significantly in the CB-MSC group only at 72 hours compared to the WJ-MSC group. For IL-4, the 1/10 ratio in the CB-MSC group decreased significantly at 24 hours, while the intracellular level was increased in all other groups. After CB-MSC/PBMC co-culture, TGF-β supernatant level decreased by 1/5 in 24 hours and increased in 72 hours. On the contrary, TGF-β supernatant level increased in the CB-MSC group at 24 hours and decreased significantly at 72 hours in 1/10 ratios (p<0.05). Conclusion: CB-MSCs and WJ-MSCs show different immunomodulatory properties. Based on these findings, it can be said that the use of WJ-MSCs is more advantageous in terms of cell therapies due to the different isolation, proliferation capacity and immunomodulatory properties of CB-MSCs.Keywords : Kordon kanı, Kordon dokusu, mezenkimal kök hücre, PKMH, ko-kültür, immunmodülasyon