- Politeknik Dergisi
- Vol: 22 Issue: 4
- Preparation of Chitosan and Glycol Chitosan Coated Magnetic Nanoparticles Loaded with Carboplatin as...
Preparation of Chitosan and Glycol Chitosan Coated Magnetic Nanoparticles Loaded with Carboplatin as Anticancer Drug
Authors : Ceren Atila Dinçer, Ali Mert Erdek, Ayşe Karakeçili, Nuray Yildiz
Pages : 1017-1022
Doi:10.2339/politeknik.501694
View : 16 | Download : 1
Publication Date : 2019-12-01
Article Type : Research
Abstract :Surface modified Fe3O4 nanoparticles (Fe3O4-OA) with an average diameter of 10 nm were synthesized, coated by chitosan (CS) and glycol chitosan (GCS), thus magnetic polymer nanocomposites were obtained. The magnetic nanostructures were analyzed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-Ray diffraction (XRD) and vibrating sample magnetometer (VSM). All magnetic structures synthesized in this study exhibited superparamagnetic properties. Loading carboplatin (CPt) as anticancer drug to Fe3O4-OA-GCS nanocomposites were carried out with 13.17 % drug content and 38 % encapsulation efficiency. The cytotoxicity studies were occured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on L929 mouse fibroblasts and MCF-7 human breast cancer cells. Fe3O4-OA-GCS-CPt nanocomposites showed higher cytotoxicity than free CPt on the MCF-7 cells at 50 µg/ml drug concentrations during 72 h.Keywords : Fe3O4-OA nanoparticles, chitosan / glycol chitosan coating, carboplatin, anticancer drug loading, drug release