- Turkish Journal of Medical Sciences
- Vol: 28 Issue: 4
- Investigation of the Inhibition Effects of Some Antibiotics on Human Erythrocyte Carbonic Anhydrase ...
Investigation of the Inhibition Effects of Some Antibiotics on Human Erythrocyte Carbonic Anhydrase Isozymes
Authors : T. Abdülkadir Çoban
Pages : 407-410
View : 14 | Download : 7
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :In this study, the in vitro effects of some antibiotics on carbonic anhydrase (CA) isozymes have been investigated. Human erythrocyte CA-I and CA-II isozymes were separately purified by affinity chromatography. Inhibition effects of four different antibiotics on CA isozymes were determined by using the CO 2 -Hydratase method. I 50 values of antibiotics were obtained by drawing % Activity [Actibiotic] graphs. The obtained I 50 values of cefazolin sodium, cephradine, sulbactam/cefoperazone and chloramphenicol sodium succinate were 9, 16, 19 and 48 mM on CA-I and 6, 10, 15 and 17 mM on CA-II, respectively. Antibiotics dosages should be taken into consideration because of the inhibition effect on CA which is pH regulatory enzyme in body.Keywords : Carbonic anhydrase isozymes, antibiotics, inhibition.