- Marmara Medical Journal
- Cilt: 37 Sayı: 1
- The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal...
The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells
Authors : Selda Güler Atmaca, Banu Aydın, Hulya Cabadak
Pages : 86-91
Doi:10.5472/marumj.1379868
View : 92 | Download : 97
Publication Date : 2024-01-28
Article Type : Research
Abstract :Objective: Muscarinic receptors (M1-M5) are members of the G protein-coupled receptor superfamily and are effective in physiological functions through G proteins. Recent studies suggested that cholinergic receptors mediate cellular activities in hematopoietic cells. The aim of this study was to investigate the potential role of mitogen-activated protein kinases (MAPK) signaling extracellular signalregulated kinases 1 and 2 (ERK1/2)/phosphorylated ERK1/ (pERK1/2) pathways in chronic myeloid leukemia (K562) cells. Materials and Methods: Chronic myeloid leukemia cells were cultured. Cells were incubated in the presence of muscarinic receptor agonist, antagonist and epidermal growth factor (EGF). To detect MAPK activation, ERK/pERK protein expression levels were determined by western blot method techniques. Results: Our study results showed that cholinergic agents and EGFs affect the MAPK pathway in the human K562 cell line. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells. Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells.Keywords : G protein coupled receptor, Carbachol, Epidermal growth factor, Mitogen-activated protein kinase, Chronic myeloid leukemia